MPC Member Publications

This database contains a listing of population studies publications written by MPC Members. Anyone can add a publication by an MPC student, faculty, or staff member to this database; new citations will be reviewed and approved by MPC administrators.

Full Citation

Title: Performance of commercial tests for molecular detection of Shiga toxin-producing Escherichia coli (STEC): A systematic review and meta-analysis protocol

Citation Type: Miscellaneous

Publication Year: 2019

ISSN: 20446055

DOI: 10.1136/bmjopen-2018-025950

PMID: 30850413

Abstract: Introduction Rapid detection of Shiga toxin-producing Escherichia coli (STEC) enables appropriate treatment. Numerous commercially available molecular tests exist, but they vary in clinical performance. This systematic review aims to synthesise available evidence to compare the clinical performance of enzyme immunoassay (EIA) and nucleic acid amplification tests (NAATs) for the detection of STEC. Methods and analysis The following databases will be searched employing a standardised search strategy: Medline, Embase, Cochrane CENTRAL Register of Controlled Trials, Cochrane Database of Systematic Reviews, PubMed, Scopus and Web of Science. Grey literature will be searched under advice from a medical librarian. Independent reviewers will screen titles, abstracts and full texts of retrieved studies for relevant studies. Data will be extracted independently by two reviewers, using a piloted template. Quality Assessment of Diagnostic Accuracy Studies-2 will be employed to assess the risk of bias of individual studies, and the quality of evidence will be assessed with the Grading of Recommendations Assessment, Development and Evaluation approach. A bivariate random-effects model will be used to meta-analyse the sensitivity and specificity of commercial STEC diagnostic tests, and a hierarchical summary receiver operator characteristic curve will be constructed. Studies of single test accuracy of EIA and NAATs and studies of comparative accuracy will be analysed separately. Ethics and dissemination Ethics approval was not required for this systematic review and meta-analysis. Findings will be disseminated in conferences, through a peer-reviewed journal and via personal interactions with relevant stakeholders. PROSPERO registration number CRD42018099119.

Url: https://pubmed.ncbi.nlm.nih.gov/30850413/

User Submitted?: No

Authors: Tarr, Gillian A.M.; Lin, Chu Yang; Lorenzetti, Diane L.; Chui, Linda; Tarr, Phillip I.; Hartling, Lisa; Vandermeer, Ben; Freedman, Stephen B.

Publisher: BMJ Publishing Group

Countries:

IPUMS NHGIS NAPP IHIS ATUS Terrapop